Coding

Part:BBa_K4760004:Design

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-09)


Lpp-OmpA-2xEAAAAK-His10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Lpp'OmpA is a variant of OmpA protein originated from E.coli. K12 genome. The Lpp'OmpA and GGGGS-His10 parts were both synthesized in our project to make the codon usage match BL21 strain preference.

References